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验证数据展示

  • WB analysis using 20820-1-AP

    WB analysis using 20820-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20820-1-AP (PTDSS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 20820-1-AP

    IF Staining of HepG2 using 20820-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PTDSS1 antibody (20820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PTDSS1 Polyclonal antibody

PTDSS1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20820-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

PSS1, PSSA, PSS-1, Phosphatidylserine synthase 1, KIAA0024

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, unboiled HEK-293 cells, HeLa cells, unboiled HeLa cells
Positive IF/ICC detected inHepG2 cells

It is best to use unboiled samples for detection, for membrane proteins are hydrophobic and tend to aggregate when boiled at high temperatures.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20820-1-AP targets PTDSS1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PTDSS1 fusion protein Ag14170 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylserine synthase 1
别名 PSS1, PSSA, PSS-1, Phosphatidylserine synthase 1, KIAA0024
计算分子量 473 aa, 56 kDa
观测分子量38~40 kDa
GenBank蛋白编号BC002376
基因名称 PTDSS1
Gene ID (NCBI) 9791
RRIDAB_3669350
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48651
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTDSS1 gene encodes the enzyme phosphatidylserine synthase 1 (PSS1), which catalyzes the production of phosphatidylserine (PS), a membrane phospholipid involved in different cellular processes such as development, cell signaling, apoptosis, and blood coagulation (PMID: 35224839). PTDSS1 is highly enriched in mitochondria-associated membranes (MAM) and is largely excluded from the bulk of the endoplasmic reticulum (ER). Low apparent molecular mass of 40 kDa compared with the size predicted was attributed to the exceptionally high content of hydrophobic amino acids in PSS1 (PMID: 10938271).

实验方案

Product Specific Protocols
WB protocol for PTDSS1 antibody 20820-1-APDownload protocol
IF protocol for PTDSS1 antibody 20820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols