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验证数据展示

  • IF Staining of Hela using 83421-1-RR

    IF Staining of Hela using 83421-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Hela cells using PTDSS1 antibody (83421-1-RR, Clone: 240007G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 83421-1-RR

    FC experiment of HepG2 using 83421-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PTDSS1 Recombinant antibody (83421-1-RR, Clone:240007G3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83421-1-RR

    Affinity and Kinetic Characterization of 83421-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83421-1-RR against Human PTDSS1 were performed. The affinity constant is below 1 pM.

PTDSS1 Recombinant antibody

PTDSS1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83421-1-RR

产品说明书

CloneNo. 240007G3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

PSS1, PSS-1, Phosphatidylserine synthase 1, KIAA0024, EC:2.7.8.29

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHela cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83421-1-RR targets PTDSS1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 PTDSS1 fusion protein Ag14170 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphatidylserine synthase 1
别名 PSS1, PSS-1, Phosphatidylserine synthase 1, KIAA0024, EC:2.7.8.29
计算分子量 473 aa, 56 kDa
GenBank蛋白编号BC002376
基因名称 PTDSS1
Gene ID (NCBI) 9791
RRIDAB_3671070
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP48651
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphatidylserine Synthase-1 (PTDSS1 or PSS1) is one of two enzymes involved in the production of phosphatidylserine (PS), which is a quantitatively minor, but physiologically important phospholipid in mammalian cells (PMID: 24241535). Mice lacking either Ptdss1 or Ptdss2 are viable, phenotypically normal and fertile, indicating that, in mice, PSS1 and PSS2 can compensate for each other (PMID: 18343815).

实验方案

Product Specific Protocols
IF protocol for PTDSS1 antibody 83421-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols