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PTER Polyclonal antibody

PTER Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11191-1-AP

Print datasheet

Synonyms

RPR 1, Phosphotriesterase-related protein, phosphotriesterase related, Parathion hydrolase-related protein, hPHRP

验证数据展示

  • IHC staining of human ovary tumor using 11191-1-AP

    IHC staining of human ovary tumor using 11191-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11191-1-AP (PTER antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HEK-293 using 11191-1-AP

    IF Staining of HEK-293 using 11191-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 11191-1-AP (PTER antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 11191-1-AP

    WB analysis using 11191-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11191-1-AP (PTER antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, mouse thymus tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11191-1-AP targets PTER in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PTER fusion protein Ag1675 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphotriesterase related
Synonyms RPR 1, Phosphotriesterase-related protein, phosphotriesterase related, Parathion hydrolase-related protein, hPHRP
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 33 kDa, 39 kDa
GenBank Accession NumberBC015092
Gene Symbol PTER
Gene ID (NCBI) 9317
RRIDAB_2174190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BW5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTER antibody 11191-1-APDownload protocol
IHC protocol for PTER antibody 11191-1-APDownload protocol
IF protocol for PTER antibody 11191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols