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验证数据展示

  • WB analysis of mouse brain using 24761-1-AP

    WB analysis of mouse brain using 24761-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24761-1-AP (PTGER3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 24761-1-AP

    IHC staining of human brain using 24761-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24761-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human brain using 24761-1-AP

    IHC staining of human brain using 24761-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24761-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 40x lens).

PTGER3 Polyclonal antibody

PTGER3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24761-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

EP3, EP3 I, EP3 II, EP3 III, EP3 IV, EP3e, PGE receptor EP3 subtype, PGE2 R, PGE2 receptor EP3 subtype, Prostanoid EP3 receptor, PTGER3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

24761-1-AP targets PTGER3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen PTGER3 fusion protein Ag21542 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prostaglandin E receptor 3 (subtype EP3)
Synonyms EP3, EP3 I, EP3 II, EP3 III, EP3 IV, EP3e, PGE receptor EP3 subtype, PGE2 R, PGE2 receptor EP3 subtype, Prostanoid EP3 receptor, PTGER3
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC024229
Gene Symbol PTGER3
Gene ID (NCBI) 5733
RRIDAB_2879710
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43115
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTGER3 antibody 24761-1-APDownload protocol
IHC protocol for PTGER3 antibody 24761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB

Dev Cell

Chromatin remodeling of prostaglandin signaling in smooth muscle enables mouse embryo passage through the female reproductive tract

Authors - Qiliang Xin
View Article
human,mouseWB

Cell Metab

m6A mRNA methylation in brown fat regulates systemic insulin sensitivity via an inter-organ prostaglandin signaling axis independent of UCP1

Authors - Ling Xiao
View Article