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验证数据展示

  • WB analysis of mouse brain using 24761-1-AP

    WB analysis of mouse brain using 24761-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24761-1-AP (PTGER3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 24761-1-AP

    IHC staining of human brain using 24761-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24761-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human brain using 24761-1-AP

    IHC staining of human brain using 24761-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24761-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 40x lens).

PTGER3 Polyclonal antibody

PTGER3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24761-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

EP3, EP3 I, EP3 II, EP3 III, EP3 IV, EP3e, PGE receptor EP3 subtype, PGE2 R, PGE2 receptor EP3 subtype, Prostanoid EP3 receptor, PTGER3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

24761-1-AP targets PTGER3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 PTGER3 fusion protein Ag21542 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 prostaglandin E receptor 3 (subtype EP3)
别名 EP3, EP3 I, EP3 II, EP3 III, EP3 IV, EP3e, PGE receptor EP3 subtype, PGE2 R, PGE2 receptor EP3 subtype, Prostanoid EP3 receptor, PTGER3
计算分子量 43 kDa
观测分子量 48 kDa
GenBank蛋白编号BC024229
基因名称 PTGER3
Gene ID (NCBI) 5733
RRIDAB_2879710
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43115
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTGER3 antibody 24761-1-APDownload protocol
IHC protocol for PTGER3 antibody 24761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB

Dev Cell

Chromatin remodeling of prostaglandin signaling in smooth muscle enables mouse embryo passage through the female reproductive tract

Authors - Qiliang Xin
View Article
human,mouseWB

Cell Metab

m6A mRNA methylation in brown fat regulates systemic insulin sensitivity via an inter-organ prostaglandin signaling axis independent of UCP1

Authors - Ling Xiao
View Article