验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, human stomach tissue, mouse heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells, HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10881-1-AP targets PTGES2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, caenorhabditis elegans |
| Immunogen | PTGES2 fusion protein Ag1329 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | prostaglandin E synthase 2 |
| Synonyms | mPGES 2, mPGE synthase-2, Membrane-associated prostaglandin E synthase-2, GBF1, EC:5.3.99.3 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 30-35 kDa, 45 kDa |
| GenBank Accession Number | BC011613 |
| Gene Symbol | PTGES2 |
| Gene ID (NCBI) | 80142 |
| RRID | AB_2175066 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H7Z7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PTGES2 (Prostaglandin E synthase 2) catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. Its predicted size is 42 kDa, but it was detected one band of 35 kDa in the prostate and two bands of 30 kDa, 50 kDa in liver by western blot (PMID:17566096). PTGES2 protein is a homotetramer, whereas two PTGES2 molecules each form a strongly interacting dimer(PMID:19268562).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTGES2 antibody 10881-1-AP | Download protocol |
| IHC protocol for PTGES2 antibody 10881-1-AP | Download protocol |
| IF protocol for PTGES2 antibody 10881-1-AP | Download protocol |
| IP protocol for PTGES2 antibody 10881-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain Behav Immun Elevated β-secretase 1 expression mediates CD4+ T cell dysfunction via PGE2 signalling in Alzheimer's disease. | ||
Int Immunopharmacol Curc-mPEG454, a PEGylated curcumin derivative, as a multi-target anti-fibrotic prodrug. | ||
Tumour Biol Prostaglandin E2 (PGE2) promotes proliferation and invasion by enhancing SUMO-1 activity via EP4 receptor in endometrial cancer.
| ||
J Neuroimmunol Prostaglandin E2 increases the expression of cyclooxygenase-2 in cultured rat microglia. | ||
Int J Radiat Biol Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field. | ||
Oncol Lett Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes invasion in endometrial cancer. |
