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PTGIS Polyclonal antibody

PTGIS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 27061-1-AP

Print datasheet

Synonyms

CYP8, CYP8A1, PGIS, Prostacyclin synthase, Prostaglandin I2 synthase, PTGI, PTGIS

验证数据展示

  • WB analysis using 27061-1-AP

    WB analysis using 27061-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27061-1-AP (PTGIS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 27061-1-AP

    IHC staining of mouse liver using 27061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27061-1-AP (PTGIS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

  • IHC staining of mouse liver using 27061-1-AP

    IHC staining of mouse liver using 27061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27061-1-AP (PTGIS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

  • IF Staining of Saos-2 using 27061-1-AP

    IF Staining of Saos-2 using 27061-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using PTGIS antibody (27061-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse lung tissue, mouse testis tissue, rat lung tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSaos-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27061-1-AP targets PTGIS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PTGIS fusion protein Ag25817 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prostaglandin I2 (prostacyclin) synthase
Synonyms CYP8, CYP8A1, PGIS, Prostacyclin synthase, Prostaglandin I2 synthase, PTGI, PTGIS
Calculated Molecular Weight 500 aa, 57 kDa
Observed Molecular Weight50-57 kDa
GenBank Accession NumberBC101809
Gene Symbol PTGIS
Gene ID (NCBI) 5740
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16647
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTGIS antibody 27061-1-APDownload protocol
IHC protocol for PTGIS antibody 27061-1-APDownload protocol
IF protocol for PTGIS antibody 27061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols