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PTGIS Polyclonal antibody

PTGIS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27061-1-AP

Print datasheet

Synonyms

PGIS, Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.4, EC:4.2.1.152, CYP8A1

验证数据展示

  • WB analysis using 27061-1-AP

    WB analysis using 27061-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27061-1-AP (PTGIS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 27061-1-AP

    IHC staining of mouse liver using 27061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27061-1-AP (PTGIS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

  • IHC staining of mouse liver using 27061-1-AP

    IHC staining of mouse liver using 27061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27061-1-AP (PTGIS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

  • IF Staining of Saos-2 using 27061-1-AP

    IF Staining of Saos-2 using 27061-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using PTGIS antibody (27061-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse lung tissue, mouse testis tissue, rat lung tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSaos-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27061-1-AP targets PTGIS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PTGIS fusion protein Ag25817 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prostaglandin I2 (prostacyclin) synthase
Synonyms PGIS, Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.4, EC:4.2.1.152, CYP8A1
Calculated Molecular Weight 500 aa, 57 kDa
Observed Molecular Weight50-57 kDa
GenBank Accession NumberBC101809
Gene Symbol PTGIS
Gene ID (NCBI) 5740
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16647
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTGIS antibody 27061-1-APDownload protocol
IHC protocol for PTGIS antibody 27061-1-APDownload protocol
IF protocol for PTGIS antibody 27061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols