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PTH1R Polyclonal antibody

PTH1R Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24275-1-AP

Print datasheet

Synonyms

parathyroid hormone 1 receptor, PFE, PTH/PTHr receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTH1R, PTHR, PTHR1

验证数据展示

  • WB analysis of U2OS using 24275-1-AP

    WB analysis of U2OS using 24275-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 24275-1-AP (PTH1R antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 24275-1-AP

    IHC staining of mouse kidney using 24275-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24275-1-AP (PTH1R antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inU2OS cells, HepG2 cells, mouse liver tissue
Positive IHC detected inmouse kidney tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24275-1-AP targets PTH1R in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PTH1R fusion protein Ag17577 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name parathyroid hormone 1 receptor
Synonyms parathyroid hormone 1 receptor, PFE, PTH/PTHr receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTH1R, PTHR, PTHR1
Calculated Molecular Weight 593 aa, 66 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC110388
Gene Symbol PTH1R
Gene ID (NCBI) 5745
RRIDAB_2879465
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ03431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTH1R is a receptor for parathyroid hormone and parathyroid hormone-related peptide (PTHrP). PTH1R is a glycoprotein with seven transmembrane domains and it has an extracellular N-terminus region and an intracellular C-terminus region. The activity of PTH1R is mediated by G proteins which activate adenylyl cyclase. It has been reported that PTHrP and PTH1R are associated with the differentiation of bone and cartilage in the developing stages. PTH1R has several isoforms with 66/59 kDa and PTH1R can form a 120 kDa dimer in the non-reducing condition. 24275-1-AP antibody detects 80 kDa protein which is similar to the results reported in publications.(PMID: 19507249, 27160269)

实验方案

Product Specific Protocols
WB protocol for PTH1R antibody 24275-1-APDownload protocol
IHC protocol for PTH1R antibody 24275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols