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验证数据展示

  • FC experiment of BxPC-3 using 14166-1-AP

    FC experiment of BxPC-3 using 14166-1-AP

    1x10^6 BxPC-3 cells were intracellularly stained with 0.4 ug PTH2R Polyclonal antibody (14166-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of L02 using 14166-1-AP

    WB analysis of L02 using 14166-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 14166-1-AP

    IHC staining of human pancreas cancer using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas cancer using 14166-1-AP

    IHC staining of human pancreas cancer using 14166-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 40x lens).

PTH2R Polyclonal antibody

PTH2R Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14166-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

PTHR2, PTH2 receptor, parathyroid hormone 2 receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, BxPC-3 cells, human testis tissue, mouse liver tissue, mouse pancreas tissue, mouse testis tissue
Positive IHC detected inhuman pancreas cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inBxPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14166-1-AP targets PTH2R in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PTH2R fusion protein Ag5364 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 parathyroid hormone 2 receptor
别名 PTHR2, PTH2 receptor, parathyroid hormone 2 receptor
计算分子量 550 aa, 62 kDa
观测分子量 62-66 kDa
GenBank蛋白编号BC036811
基因名称 PTH2R
Gene ID (NCBI) 5746
RRIDAB_2253212
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49190
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTH2R antibody 14166-1-APDownload protocol
IHC protocol for PTH2R antibody 14166-1-APDownload protocol
FC protocol for PTH2R antibody 14166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols