验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, U-87 MG cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 3 publications below |
产品信息
27117-1-AP targets PTN in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig |
| 免疫原 | PTN fusion protein Ag26004 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pleiotrophin |
| 别名 | pleiotrophin, Heparin-binding neurite outgrowth-promoting factor, Heparin-binding growth-associated molecule, Heparin-binding growth factor 8, Heparin-binding brain mitogen |
| 计算分子量 | 18 kDa |
| 观测分子量 | 17-19 kDa |
| GenBank蛋白编号 | BC005916 |
| 基因名称 | PTN |
| Gene ID (NCBI) | 5764 |
| RRID | AB_2880763 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P21246 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pleiotrophin (PTN) is a multifunctional, cationic, glycosaminoglycan-binding cytokine and growth factor involved in numerous physiological and pathological processes, including tissue repair and inflammation-related diseases. PTN is highly upregulated in the brain in different disorders characterized by overt neuroinflammation such as neurodegenerative diseases, drug addiction, traumatic injury, and ischemia. PTN is important for CNS repair and for survival and differentiation of dopaminergic neurons.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTN antibody 27117-1-AP | Download protocol |
| IHC protocol for PTN antibody 27117-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Biosci Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos | ||
Oncol Rep Luteolin suppresses colorectal cancer cell metastasis via regulation of the miR‑384/pleiotrophin axis. | ||
