验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, U-87 MG cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 3 publications below |
产品信息
27117-1-AP targets PTN in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Immunogen | PTN fusion protein Ag26004 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | pleiotrophin |
| Synonyms | pleiotrophin, Heparin-binding neurite outgrowth-promoting factor, Heparin-binding growth-associated molecule, Heparin-binding growth factor 8, Heparin-binding brain mitogen |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 17-19 kDa |
| GenBank Accession Number | BC005916 |
| Gene Symbol | PTN |
| Gene ID (NCBI) | 5764 |
| RRID | AB_2880763 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P21246 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pleiotrophin (PTN) is a multifunctional, cationic, glycosaminoglycan-binding cytokine and growth factor involved in numerous physiological and pathological processes, including tissue repair and inflammation-related diseases. PTN is highly upregulated in the brain in different disorders characterized by overt neuroinflammation such as neurodegenerative diseases, drug addiction, traumatic injury, and ischemia. PTN is important for CNS repair and for survival and differentiation of dopaminergic neurons.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTN antibody 27117-1-AP | Download protocol |
| IHC protocol for PTN antibody 27117-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Biosci Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos | ||
Oncol Rep Luteolin suppresses colorectal cancer cell metastasis via regulation of the miR‑384/pleiotrophin axis. | ||
