验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
产品信息
11508-1-AP targets PTP4A1/PRL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | PTP4A1/PRL1 fusion protein Ag2067 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase type IVA, member 1 |
| 别名 | HH72, PRL 1, PRL1, PTP(CAAX1), PTP(CAAXI), PTP4A1, PTP4A1/PRL1, PTPCAAX1 |
| 计算分子量 | 20 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC023975 |
| 基因名称 | PTP4A1 |
| Gene ID (NCBI) | 7803 |
| RRID | AB_2237951 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q93096 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PTP4A1(protein tyrosine phosphatase type IVA, member 1), also named as PRL1, HH72, PRL-1, PTP(CAAX1), PTPCAAX1, DKFZp779M0721, belongs to the protein-tyrosine phosphatase family, which play an important role in maintaining appropriate tyrosine phosphorylation levels in the cell during development and tissue regeneration (PMID:19341304). PTP4A1 is a PTPase and that it is highly expressed in growing hepatic cells and some tumor cell lines and is located primarily in the cell nucleus, and its overexpression modulates the growth of cells (PMID:8196618). PTP4A1 can exist as dimer, trimer and higher oligomers in vivo (PMID: 15571731, 18224294).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTP4A1/PRL1 antibody 11508-1-AP | Download protocol |
| IHC protocol for PTP4A1/PRL1 antibody 11508-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Signal The adaptor SH2B1 and the phosphatase PTP4A1 regulate the phosphorylation of cytohesin-2 in myelinating Schwann cells in mice. | ||
Oncotarget Oncogenic ALK regulates EMT in non-small cell lung carcinoma through repression of the epithelial splicing regulatory protein 1. | ||
Int J Oncol MicroRNA-1271 functions as a metastasis and epithelial-mesenchymal transition inhibitor in human HCC by targeting the PTP4A1/c-Src axis.
| ||
PLoS One MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1. | ||
Int J Cancer ERα inhibits mesenchymal and amoeboidal movement of liver cancer cell via Gα12
|