• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HEK-293 using 24570-1-AP

    WB analysis of HEK-293 using 24570-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24570-1-AP (PTPN11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 24570-1-AP

    IHC staining of human gliomas using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24570-1-AP (PTPN11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IHC staining of human gliomas using 24570-1-AP

    IHC staining of human gliomas using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24570-1-AP (PTPN11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IHC staining of human skeletal muscle using 24570-1-AP

    IHC staining of human skeletal muscle using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24570-1-AP (PTPN11 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IHC staining of human skeletal muscle using 24570-1-AP

    IHC staining of human skeletal muscle using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24570-1-AP (PTPN11 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IF Staining of MCF-7 using 24570-1-AP

    IF Staining of MCF-7 using 24570-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PTPN11/SHP2 antibody (24570-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

SHP2 Polyclonal antibody

SHP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24570-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

PTPN11/SHP2, PTPN11, NS1, NS 1, EC:3.1.3.48

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, U-937 cells
Positive IHC detected inhuman gliomas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PTPN11 Ready-To-Use IHC Kit. PTPN11 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24570-1-AP targets SHP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原 SHP2 fusion protein Ag21472 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 11
别名 PTPN11/SHP2, PTPN11, NS1, NS 1, EC:3.1.3.48
计算分子量 597 aa, 68 kDa
观测分子量 68 kda
GenBank蛋白编号BC008692
基因名称 PTPN11
Gene ID (NCBI) 5781
RRIDAB_2879613
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ06124
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHP2 antibody 24570-1-APDownload protocol
IHC protocol for SHP2 antibody 24570-1-APDownload protocol
IF protocol for SHP2 antibody 24570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Oncol Lett

Curcumin inhibits proliferation of hepatocellular carcinoma cells by blocking PTPN1 and PTPN11 expression

Authors - Jingru Zhang
View Article
humanWB

Heliyon

RNA-binding protein THUMPD2 inhibits proliferation and promotes metastasis in epithelial ovarian cancer

Authors - Minhui Hua
View Article