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验证数据展示

  • WB analysis of HEK-293 using 24570-1-AP

    WB analysis of HEK-293 using 24570-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24570-1-AP (PTPN11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 24570-1-AP

    IHC staining of human gliomas using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24570-1-AP (PTPN11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IHC staining of human gliomas using 24570-1-AP

    IHC staining of human gliomas using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24570-1-AP (PTPN11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IHC staining of human skeletal muscle using 24570-1-AP

    IHC staining of human skeletal muscle using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24570-1-AP (PTPN11 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IHC staining of human skeletal muscle using 24570-1-AP

    IHC staining of human skeletal muscle using 24570-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24570-1-AP (PTPN11 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IF Staining of MCF-7 using 24570-1-AP

    IF Staining of MCF-7 using 24570-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PTPN11/SHP2 antibody (24570-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

SHP2 Polyclonal antibody

SHP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24570-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PTPN11/SHP2, PTPN11, NS1, NS 1, EC:3.1.3.48

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, U-937 cells
Positive IHC detected inhuman gliomas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PTPN11 Ready-To-Use IHC Kit. PTPN11 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24570-1-AP targets SHP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen SHP2 fusion protein Ag21472 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 11
Synonyms PTPN11/SHP2, PTPN11, NS1, NS 1, EC:3.1.3.48
Calculated Molecular Weight 597 aa, 68 kDa
Observed Molecular Weight 68 kda
GenBank Accession NumberBC008692
Gene Symbol PTPN11
Gene ID (NCBI) 5781
RRIDAB_2879613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06124
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHP2 antibody 24570-1-APDownload protocol
IHC protocol for SHP2 antibody 24570-1-APDownload protocol
IF protocol for SHP2 antibody 24570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Oncol Lett

Curcumin inhibits proliferation of hepatocellular carcinoma cells by blocking PTPN1 and PTPN11 expression

Authors - Jingru Zhang
View Article
humanWB

Heliyon

RNA-binding protein THUMPD2 inhibits proliferation and promotes metastasis in epithelial ovarian cancer

Authors - Minhui Hua
View Article