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  • Featured Product
  • KD/KO Validated

PTPN11/SHP2 Recombinant antibody

PTPN11/SHP2 Uni-rAbTM Recombinant Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5L23

Cat no : 82503-1-RR

Print datasheet

Synonyms

PTPN11, SHP2, NS 1, EC:3.1.3.48, CFC

验证数据展示

  • WB analysis using 82503-1-RR

    WB analysis using 82503-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82503-1-RR (PTPN11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82503-1-RR

    WB analysis of HeLa using 82503-1-RR

    WB result of SHP2 antibody (82503-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHP2 transfected HeLa cells.

  • IHC staining of rat heart using 82503-1-RR

    IHC staining of rat heart using 82503-1-RR

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 82503-1-RR (SHP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IHC staining of rat heart using 82503-1-RR

    IHC staining of rat heart using 82503-1-RR

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 82503-1-RR (SHP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IP experiment of HEK-293 using 82503-1-RR

    IP experiment of HEK-293 using 82503-1-RR

    IP result of anti-PTPN11/SHP2 (IP:82503-1-RR, 4ug; Detection:82503-1-RR 1:8000) with HEK-293 cells lysate 1280 ug.

  • Affinity and Kinetic Characterization of 82503-1-RR

    Affinity and Kinetic Characterization of 82503-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82503-1-RR against Human PTPN11/SHP2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, A549 cells, NIH/3T3 cells, HSC-T6 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inrat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PTPN11 Ready-To-Use IHC Kit. PTPN11 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82503-1-RR targets PTPN11/SHP2 in WB, IP, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen PTPN11/SHP2 fusion protein Ag13660 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 11
Synonyms PTPN11, SHP2, NS 1, EC:3.1.3.48, CFC
Calculated Molecular Weight 597 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC008692
Gene Symbol PTPN11
Gene ID (NCBI) 5781
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06124
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN11(protein tyrosine phosphatase, non-receptor type 11), also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, and MGC14433, belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML), and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PTPN11/SHP2 antibody 82503-1-RRDownload protocol
IHC protocol for PTPN11/SHP2 antibody 82503-1-RRDownload protocol
IP protocol for PTPN11/SHP2 antibody 82503-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols