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  • Featured Product
  • KD/KO Validated

SHP2 Recombinant antibody

SHP2 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

5L23

Cat no : 82503-1-RR

Print datasheet

Synonyms

PTPN11/SHP2, PTPN11, NS 1, EC:3.1.3.48, CFC

验证数据展示

  • WB analysis using 82503-1-RR

    WB analysis using 82503-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82503-1-RR (PTPN11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82503-1-RR

    WB analysis of HeLa using 82503-1-RR

    WB result of SHP2 antibody (82503-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHP2 transfected HeLa cells.

  • IHC staining of rat heart using 82503-1-RR

    IHC staining of rat heart using 82503-1-RR

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 82503-1-RR (SHP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IHC staining of rat heart using 82503-1-RR

    IHC staining of rat heart using 82503-1-RR

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 82503-1-RR (SHP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IP experiment of HEK-293 using 82503-1-RR

    IP experiment of HEK-293 using 82503-1-RR

    IP result of anti-PTPN11/SHP2 (IP:82503-1-RR, 4ug; Detection:82503-1-RR 1:8000) with HEK-293 cells lysate 1280 ug.

  • Affinity and Kinetic Characterization of 82503-1-RR

    Affinity and Kinetic Characterization of 82503-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82503-1-RR against Human PTPN11/SHP2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, A549 cells, NIH/3T3 cells, HSC-T6 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inrat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PTPN11 Ready-To-Use IHC Kit. PTPN11 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82503-1-RR targets SHP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SHP2 fusion protein Ag13660 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 11
Synonyms PTPN11/SHP2, PTPN11, NS 1, EC:3.1.3.48, CFC
Calculated Molecular Weight 597 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC008692
Gene Symbol PTPN11
Gene ID (NCBI) 5781
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06124
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN11(protein tyrosine phosphatase, non-receptor type 11), also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, and MGC14433, belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML), and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for SHP2 antibody 82503-1-RRDownload protocol
IHC protocol for SHP2 antibody 82503-1-RRDownload protocol
IP protocol for SHP2 antibody 82503-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols