• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

SHP2 Polyclonal antibody, PBS Only

SHP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20145-1-PBS

Print datasheet

Synonyms

PTPN11, NS1, NS 1, EC:3.1.3.48, CFC

验证数据展示

  • WB analysis using 20145-1-AP (same clone as 20145-1-PBS)

    WB analysis using 20145-1-AP (same clone as 20145-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20145-1-AP (PTPN11/SHP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 20145-1-AP (same clone as 20145-1-PBS)

    IP experiment of HEK-293 using 20145-1-AP (same clone as 20145-1-PBS)

    IP result of anti-PTPN11/SHP2 (IP:20145-1-AP, 4ug; Detection:20145-1-AP 1:10000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 20145-1-AP (same clone as 20145-1-PBS)

    IHC staining of human breast cancer using 20145-1-AP (same clone as 20145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20145-1-AP (SHP2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IHC staining of human breast cancer using 20145-1-AP (same clone as 20145-1-PBS)

    IHC staining of human breast cancer using 20145-1-AP (same clone as 20145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20145-1-AP (SHP2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IHC staining of human gliomas using 20145-1-AP (same clone as 20145-1-PBS)

    IHC staining of human gliomas using 20145-1-AP (same clone as 20145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20145-1-AP (SHP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IHC staining of human gliomas using 20145-1-AP (same clone as 20145-1-PBS)

    IHC staining of human gliomas using 20145-1-AP (same clone as 20145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20145-1-AP (SHP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PTPN11 antibody included
  • IF Staining of HeLa using 20145-1-AP (same clone as 20145-1-PBS)

    IF Staining of HeLa using 20145-1-AP (same clone as 20145-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHP2 antibody (20145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20145-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PTPN11 Ready-To-Use IHC Kit. PTPN11 primary antibody included.

产品信息

20145-1-PBS targets SHP2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SHP2 fusion protein Ag13660 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 11
Synonyms PTPN11, NS1, NS 1, EC:3.1.3.48, CFC
Calculated Molecular Weight 597 aa, 68 kDa
Observed Molecular Weight 68-75 kDa
GenBank Accession NumberBC008692
Gene Symbol PTPN11
Gene ID (NCBI) 5781
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06124
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PTPN11(protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.