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验证数据展示

  • WB analysis using 31215-1-AP

    WB analysis using 31215-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31215-1-AP (PTPN12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 31215-1-AP

    IF Staining of A431 using 31215-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PTPN12 antibody (31215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of U-251 using 31215-1-AP

    IF Staining of U-251 using 31215-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PTPN12 antibody (31215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PTPN12 Polyclonal antibody

PTPN12 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31215-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

PTPG1, PTP PEST

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IF/ICC detected inA431 cells, U-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31215-1-AP targets PTPN12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen PTPN12 fusion protein Ag35382 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 12
Synonyms PTPG1, PTP PEST
Calculated Molecular Weight88kDa
Observed Molecular Weight110-125 kDa
GenBank Accession NumberNM_002835.3
Gene Symbol PTPN12
Gene ID (NCBI) 5782
RRIDAB_3669905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ05209
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa.

实验方案

Product Specific Protocols
WB protocol for PTPN12 antibody 31215-1-APDownload protocol
IF protocol for PTPN12 antibody 31215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols