验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, MCF-7 cells |
Positive IF/ICC detected in | A431 cells, U-251 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
31215-1-AP targets PTPN12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | PTPN12 fusion protein Ag35382 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | protein tyrosine phosphatase, non-receptor type 12 |
别名 | PTPG1, PTP PEST |
计算分子量 | 88kDa |
观测分子量 | 110-125 kDa |
GenBank蛋白编号 | NM_002835.3 |
基因名称 | PTPN12 |
Gene ID (NCBI) | 5782 |
RRID | AB_3669905 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity Purification |
UNIPROT ID | Q05209 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for PTPN12 antibody 31215-1-AP | Download protocol |
IF protocol for PTPN12 antibody 31215-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |