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PTPN12 Recombinant antibody

PTPN12 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240983C9

Cat no : 83851-6-RR

Print datasheet

Synonyms

PTPG1, PTP PEST, 240983C9

验证数据展示

  • WB analysis using 83851-6-RR

    WB analysis using 83851-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83851-6-RR (PTPN12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83851-6-RR

    IHC staining of human ovary cancer using 83851-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83851-6-RR (PTPN12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83851-6-RR

    Affinity and Kinetic Characterization of 83851-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83851-6-RR against Human PTPN12 were performed. The affinity constant is 48.4 pM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, HEK-293T cells, A549 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83851-6-RR targets PTPN12 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen PTPN12 fusion protein Ag35382 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 12
Synonyms PTPG1, PTP PEST, 240983C9
Calculated Molecular Weight88kDa
Observed Molecular Weight110-125 kDa
GenBank Accession NumberNM_002835.3
Gene Symbol PTPN12
Gene ID (NCBI) 5782
RRIDAB_3671436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ05209
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa.

实验方案

Product Specific Protocols
WB protocol for PTPN12 antibody 83851-6-RRDownload protocol
IHC protocol for PTPN12 antibody 83851-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols