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PTPN14 Polyclonal antibody

PTPN14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18053-1-AP

Print datasheet

Synonyms

PEZ, PTP36, PTPN14

验证数据展示

  • WB analysis using 18053-1-AP

    WB analysis using 18053-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18053-1-AP (PTPN14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 18053-1-AP

    IHC staining of human placenta using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 18053-1-AP

    IHC staining of human placenta using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of HeLa using 18053-1-AP

    WB analysis of HeLa using 18053-1-AP

    WB result of PTPN14 antibody (18053-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN14 transfected HeLa cells.

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inhuman placenta tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18053-1-AP targets PTPN14 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitycanine
Immunogen PTPN14 fusion protein Ag12571 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 14
Synonyms PEZ, PTP36, PTPN14
Calculated Molecular Weight 1187 aa, 135 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC101754
Gene Symbol PTPN14
Gene ID (NCBI) 5784
RRIDAB_2284584
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15678
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY).

实验方案

Product Specific Protocols
WB protocol for PTPN14 antibody 18053-1-APDownload protocol
IHC protocol for PTPN14 antibody 18053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
canineWB

Biologicals

CDC20 is a potential target gene to inhibit the tumorigenesis of MDCK cells

Authors - Zhenbin Liu
View Article