验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, Raji cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11783-1-AP targets PTPN22 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PTPN22 fusion protein Ag2397 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, non-receptor type 22 (lymphoid) |
| 别名 | PEP, LYP2, LYP1, LyP, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP |
| 计算分子量 | 82 kDa, 92 kDa |
| 观测分子量 | 100-110 kDa |
| GenBank蛋白编号 | BC017785 |
| 基因名称 | PTPN22 |
| Gene ID (NCBI) | 26191 |
| RRID | AB_2173378 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2R2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PTPN22(Tyrosine-protein phosphatase non-receptor type 22) is also named as LyP,PEP,PTPN8.It belongs to the protein-tyrosine phosphatase family and non-receptor class 4 subfamily.It acts as negative regulator of T-cell receptor (TCR) signaling.Defects in PTPN22 are a cause of susceptibility to systemic lupus erythematosus (SLE). It has some isoforms produced by alternative splicing with calculated molecular mass of 79-92 kDa, and apparent molecular mass of 100-110 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTPN22 antibody 11783-1-AP | Download protocol |
| IHC protocol for PTPN22 antibody 11783-1-AP | Download protocol |
| IP protocol for PTPN22 antibody 11783-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Med NLR Nod1 signaling promotes survival of BCR-engaged mature B cells through up-regulated Nod1 as a positive outcome. | ||
Sci Signal The adaptor protein TRAF3 inhibits interleukin-6 receptor signaling in B cells to limit plasma cell development. | ||
Pflugers Arch Anandamide produced by Ca(2+)-insensitive enzymes induces excitation in primary sensory neurons. |
