PTPN22 Polyclonal antibody

PTPN22 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11783-1-AP

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Synonyms

Lymphoid phosphatase, LYP, LYP1, LYP2, PEP, PTPN22, PTPN8



经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11783-1-AP targets PTPN22 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen PTPN22 fusion protein Ag2397 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
Synonyms Lymphoid phosphatase, LYP, LYP1, LYP2, PEP, PTPN22, PTPN8
Calculated Molecular Weight 82 kDa, 92 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC017785
Gene Symbol PTPN22
Gene ID (NCBI) 26191
RRIDAB_2173378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2R2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN22(Tyrosine-protein phosphatase non-receptor type 22) is also named as LyP,PEP,PTPN8.It belongs to the protein-tyrosine phosphatase family and non-receptor class 4 subfamily.It acts as negative regulator of T-cell receptor (TCR) signaling.Defects in PTPN22 are a cause of susceptibility to systemic lupus erythematosus (SLE). It has some isoforms produced by alternative splicing with calculated molecular mass of 79-92 kDa, and apparent molecular mass of 100-110 kDa.

实验方案

Product Specific Protocols
WB protocol for PTPN22 antibody 11783-1-APDownload protocol
IHC protocol for PTPN22 antibody 11783-1-APDownload protocol
IP protocol for PTPN22 antibody 11783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Exp Med

NLR Nod1 signaling promotes survival of BCR-engaged mature B cells through up-regulated Nod1 as a positive outcome.

Authors - Kyoko Hayakawa
mouseWB,IP

Sci Signal

The adaptor protein TRAF3 inhibits interleukin-6 receptor signaling in B cells to limit plasma cell development.

Authors - Wai W Lin
humanWB

Sci Rep

TRAF3 enhances TCR signaling by regulating the inhibitors Csk and PTPN22.

Authors - Alicia M Wallis
ratIF

Pflugers Arch

Anandamide produced by Ca(2+)-insensitive enzymes induces excitation in primary sensory neurons.

Authors - Varga Angelika A