• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PTPN5 Polyclonal antibody

PTPN5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14515-1-AP

Print datasheet

Synonyms

PTPN5, PTPSTEP, STEP

验证数据展示

  • WB analysis of HL-60 using 14515-1-AP

    WB analysis of HL-60 using 14515-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14515-1-AP (PTPN5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 14515-1-AP

    IHC staining of mouse spleen using 14515-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14515-1-AP (PTPN5 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of mouse spleen using 14515-1-AP

    IHC staining of mouse spleen using 14515-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14515-1-AP (PTPN5 Antibody) at dilution of 1:200 (under 10x lens).

  • IP experiment of mouse brain using 14515-1-AP

    IP experiment of mouse brain using 14515-1-AP

    IP result of anti-PTPN5 (IP:14515-1-AP, 4ug; Detection:14515-1-AP 1:1500) with mouse brain tissue lysate 1840 ug.

经过测试的应用

Positive WB detected inHL-60 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14515-1-AP targets PTPN5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen PTPN5 fusion protein Ag5974 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched)
Synonyms PTPN5, PTPSTEP, STEP
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 46-50 kDa, 61-66 kDa
GenBank Accession NumberBC064807
Gene Symbol PTPN5
Gene ID (NCBI) 84867
RRIDAB_2173548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54829
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTPN5 antibody 14515-1-APDownload protocol
IHC protocol for PTPN5 antibody 14515-1-APDownload protocol
IP protocol for PTPN5 antibody 14515-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Mol Neurobiol

The Weakened Interaction Between HECTD4 and GluN2B in Ischemic Stroke Promotes Calcium Overload and Brain Injury Through a Mechanism Involving the Decrease of GluN2B and MALT1 Ubiquitination

Authors - Yi-Yue Zhang
View Article