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验证数据展示

  • WB analysis of HL-60 using 14515-1-AP

    WB analysis of HL-60 using 14515-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14515-1-AP (PTPN5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 14515-1-AP

    IHC staining of mouse spleen using 14515-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14515-1-AP (PTPN5 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of mouse spleen using 14515-1-AP

    IHC staining of mouse spleen using 14515-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14515-1-AP (PTPN5 Antibody) at dilution of 1:200 (under 10x lens).

  • IP experiment of mouse brain using 14515-1-AP

    IP experiment of mouse brain using 14515-1-AP

    IP result of anti-PTPN5 (IP:14515-1-AP, 4ug; Detection:14515-1-AP 1:1500) with mouse brain tissue lysate 1840 ug.

PTPN5 Polyclonal antibody

PTPN5 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14515-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

PTPN5, PTPSTEP, STEP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14515-1-AP targets PTPN5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原 PTPN5 fusion protein Ag5974 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched)
别名 PTPN5, PTPSTEP, STEP
计算分子量 64 kDa
观测分子量 46-50 kDa, 61-66 kDa
GenBank蛋白编号BC064807
基因名称 PTPN5
Gene ID (NCBI) 84867
RRIDAB_2173548
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54829
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTPN5 antibody 14515-1-APDownload protocol
IHC protocol for PTPN5 antibody 14515-1-APDownload protocol
IP protocol for PTPN5 antibody 14515-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Mol Neurobiol

The Weakened Interaction Between HECTD4 and GluN2B in Ischemic Stroke Promotes Calcium Overload and Brain Injury Through a Mechanism Involving the Decrease of GluN2B and MALT1 Ubiquitination

Authors - Yi-Yue Zhang
View Article