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PTPN6 Polyclonal antibody

PTPN6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24143-1-AP

Print datasheet

Synonyms

SHP1, Protein-tyrosine phosphatase 1C, HPTP1C, Hematopoietic cell protein-tyrosine phosphatase, HCPH

验证数据展示

  • WB analysis using 24143-1-AP

    WB analysis using 24143-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 24143-1-AP (PTPN6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 24143-1-AP

    IHC staining of human ovary cancer using 24143-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 24143-1-AP (PTPN6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN6 antibody included

经过测试的应用

Positive WB detected inA431 cells, HL-60 cells, Jurkat cells, K-562 cells, Raji cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PTPN6 Ready-To-Use IHC Kit. PTPN6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24143-1-AP targets PTPN6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen PTPN6 fusion protein Ag21415 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 6
Synonyms SHP1, Protein-tyrosine phosphatase 1C, HPTP1C, Hematopoietic cell protein-tyrosine phosphatase, HCPH
Calculated Molecular Weight 597 aa, 68 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC002523
Gene Symbol PTPN6
Gene ID (NCBI) 5777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP29350
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTPN6 antibody 24143-1-APDownload protocol
IHC protocol for PTPN6 antibody 24143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols