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PTPN9 Polyclonal antibody

PTPN9 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12109-1-AP

Print datasheet

Synonyms

MEG2, PTPase MEG2, PTPMEG2, PTPN9

验证数据展示

  • WB analysis of A549 using 12109-1-AP

    WB analysis of A549 using 12109-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12109-1-AP

    IP experiment of HEK-293 using 12109-1-AP

    IP result of anti-PTPN9 (IP:12109-1-AP, 4ug; Detection:12109-1-AP 1:300) with HEK-293 cells lysate 1800ug.

  • IHC staining of human liver cancer using 12109-1-AP

    IHC staining of human liver cancer using 12109-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12109-1-AP (PTPN9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 12109-1-AP

    IHC staining of human liver cancer using 12109-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12109-1-AP (PTPN9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 12109-1-AP

    WB analysis using 12109-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, Transfected HEK-293 cells, mouse liver tissue, mouse pancreas tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12109-1-AP targets PTPN9 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen PTPN9 fusion protein Ag2754 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 9
Synonyms MEG2, PTPase MEG2, PTPMEG2, PTPN9
Calculated Molecular Weight 593 aa, 68 kDa
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC010863
Gene Symbol PTPN9
Gene ID (NCBI) 5780
RRIDAB_2877826
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43378
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN9, Tyrosine-protein phosphatase non-receptor type 9, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN9 is involved in the transfer of hydrophobic ligands or in functions of the Golgi apparatus (PMID: 19167335). MiR-96 inhibits PTPN9 expression and consequently promotes proliferation, migration and invasion of breast cancer cells (PMID:27857177). PTPN9 can be detected double bands (PMID: 22394684). PTPN9 was located in cytoplasm and nucleus in human breast cancer (PMID: 23418360).

实验方案

Product Specific Protocols
WB protocol for PTPN9 antibody 12109-1-APDownload protocol
IHC protocol for PTPN9 antibody 12109-1-APDownload protocol
IP protocol for PTPN9 antibody 12109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Ther Nucleic Acids

MicroRNA-126 Regulates Angiogenesis and Neurogenesis in a Mouse Model of Focal Cerebral Ischemia.

Authors - Meijie Qu
View Article