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  • Featured Product
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PTPN9 Polyclonal antibody

PTPN9 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30282-1-AP

Print datasheet

Synonyms

MEG2, PTPase MEG2, PTPMEG2, PTPN9

验证数据展示

  • WB analysis using 30282-1-AP

    WB analysis using 30282-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30282-1-AP (PTPN9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 30282-1-AP

    IP experiment of HEK-293 using 30282-1-AP

    IP result of anti-PTPN9 (IP:30282-1-AP, 4ug; Detection:30282-1-AP 1:1000) with HEK-293 cells lysate 1480 ug.

  • WB analysis of A549 using 30282-1-AP

    WB analysis of A549 using 30282-1-AP

    WB result of PTPN9 antibody (30282-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN9 transfected A549 cells.

  • IHC staining of human oesophagus cancer using 30282-1-AP

    IHC staining of human oesophagus cancer using 30282-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 30282-1-AP (PTPN9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, mouse pancreas tissue, HCT 116 cells, MCF-7 cells, MDA-MB-468 cells, SW480 cells, rat pancreas tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30282-1-AP targets PTPN9 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen PTPN9 fusion protein Ag31971 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 9
Synonyms MEG2, PTPase MEG2, PTPMEG2, PTPN9
Calculated Molecular Weight 593 aa, 68 kDa
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC010863
Gene Symbol PTPN9
Gene ID (NCBI) 5780
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43378
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN9, Tyrosine-protein phosphatase non-receptor type 9, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN9 is involved in the transfer of hydrophobic ligands or in functions of the Golgi apparatus (PMID: 19167335). MiR-96 inhibits PTPN9 expression and consequently promotes proliferation, migration and invasion of breast cancer cells (PMID:27857177). PTPN9 can be detected double bands (PMID: 22394684). PTPN9 was located in cytoplasm and nucleus in human breast cancer (PMID: 23418360).

实验方案

Product Specific Protocols
WB protocol for PTPN9 antibody 30282-1-APDownload protocol
IHC protocol for PTPN9 antibody 30282-1-APDownload protocol
IP protocol for PTPN9 antibody 30282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols