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PTPRCAP Polyclonal antibody

PTPRCAP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28286-1-AP

Print datasheet

Synonyms

CD45 AP, CD45 associated protein, LPAP, PTPRC associated protein, PTPRCAP

验证数据展示

  • WB analysis of Raji using 28286-1-AP

    WB analysis of Raji using 28286-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 28286-1-AP (PTPRCAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 28286-1-AP

    WB analysis using 28286-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28286-1-AP (PTPRCAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 28286-1-AP

    IHC staining of human spleen using 28286-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28286-1-AP (PTPRCAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 28286-1-AP

    IHC staining of human spleen using 28286-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28286-1-AP (PTPRCAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, Raji cells
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28286-1-AP targets PTPRCAP in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen PTPRCAP fusion protein Ag27236 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C-associated protein
Synonyms CD45 AP, CD45 associated protein, LPAP, PTPRC associated protein, PTPRCAP
Calculated Molecular Weight 206 aa, 21 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC113859
Gene Symbol PTPRCAP
Gene ID (NCBI) 5790
RRIDAB_2881105
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14761
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTPRCAP antibody 28286-1-APDownload protocol
IHC protocol for PTPRCAP antibody 28286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Discov Oncol

New immunological potential markers for triple negative breast cancer: IL18R1, CD53, TRIM, Jaw1, LTB, PTPRCAP.

Authors - Paolo Marchetti
View Article