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PTPRK Polyclonal antibody

PTPRK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28151-1-AP

Print datasheet

Synonyms

DKFZp686C2268, DKFZp779N1045, PTPK, PTPRK, R PTP kappa

验证数据展示

  • WB analysis using 28151-1-AP

    WB analysis using 28151-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28151-1-AP (PTPRK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse spleen tissue, rat spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28151-1-AP targets PTPRK in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen PTPRK fusion protein Ag27765 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, K
Synonyms DKFZp686C2268, DKFZp779N1045, PTPK, PTPRK, R PTP kappa
Calculated Molecular Weight 1439 aa, 162 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC140775
Gene Symbol PTPRK
Gene ID (NCBI) 5796
RRIDAB_2881074
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15262
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPRK (Protein Tyrosine Phosphatase Receptor Type Kappa), a member of the receptor-type protein tyrosine phosphatase family, is a transmembrane protein that regulates cell-cell contact. PTPRK is a 210 kDa precursor protein and converted by furin to a mature heterodimeric protein composed of a non-covalently attached amino-terminal extracellular subunit (E-subunit, 120 kDa) and carboxyl-terminal transmembrane subunit (P-subunit, 95 kDa) (PMID: 24882578, 16648485). Loss of PTPRK activity has been observed in pancreatic cancer, primary CNS lymphoma and melanoma, and is associated with poor survival of cancer patients, which suggest that PTPRK is a potential tumor suppressor (PMID: 23696788).

实验方案

Product Specific Protocols
WB protocol for PTPRK antibody 28151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols