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IA-2/PTPRN Polyclonal antibody

IA-2/PTPRN Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10584-1-AP

Print datasheet

Synonyms

IA-2, PTPRN, ICA512-transmembrane fragment, ICA512-N-terminal fragment, ICA512-cleaved cytosolic fragment

验证数据展示

  • WB analysis of mouse brain using 10584-1-AP

    WB analysis of mouse brain using 10584-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 10584-1-AP

    IP experiment of mouse brain using 10584-1-AP

    IP result of anti-IA-2/PTPRN (IP:10584-1-AP, 3ug; Detection:10584-1-AP 1:600) with mouse brain tissue lysate 7000ug.

  • IHC staining of human pancreas cancer using 10584-1-AP

    IHC staining of human pancreas cancer using 10584-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PTPRN antibody included
  • IF Staining of mouse pancreas using 10584-1-AP

    IF Staining of mouse pancreas using 10584-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using IA-2/PTPRN antibody (10584-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HEK-293 using 10584-1-AP

    FC experiment of HEK-293 using 10584-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human IA-2/PTPRN (10584-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse brain tissue, HEK-293 cells, mouse kidney tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy PTPRN Ready-To-Use IHC Kit. PTPRN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10584-1-AP targets IA-2/PTPRN in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen IA-2/PTPRN fusion protein Ag0923 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, N
Synonyms IA-2, PTPRN, ICA512-transmembrane fragment, ICA512-N-terminal fragment, ICA512-cleaved cytosolic fragment
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 60-65 kDa, 106-115 kDa
GenBank Accession NumberBC007713
Gene Symbol IA-2/PTPRN
Gene ID (NCBI) 5798
RRIDAB_2237983
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16849
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell).(PMID: 10027571). IA-2/PTPRN was found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.

实验方案

Product Specific Protocols
WB protocol for IA-2/PTPRN antibody 10584-1-APDownload protocol
IHC protocol for IA-2/PTPRN antibody 10584-1-APDownload protocol
IF protocol for IA-2/PTPRN antibody 10584-1-APDownload protocol
IP protocol for IA-2/PTPRN antibody 10584-1-APDownload protocol
FC protocol for IA-2/PTPRN antibody 10584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Front Cell Dev Biol

Overexpression of PTPRN Promotes Metastasis of Lung Adenocarcinoma and Suppresses NK Cell Cytotoxicity.

Authors - Xinyue Song
View Article
humanWB

J Biol Chem

Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.

Authors - Chengfeng Merriman
View Article

PLoS One

Sensitive detection of multiple islet autoantibodies in type 1 diabetes using small sample volumes by agglutination-PCR.

Authors - Felipe de Jesus Cortez
View Article
humanIHC

Cell Physiol Biochem

Prognostic Value of Phosphotyrosine Phosphatases in Hepatocellular Carcinoma.

Authors - Guangyan Zhangyuan
View Article