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PTPRS Polyclonal antibody

PTPRS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29415-1-AP

Print datasheet

Synonyms

Receptor-type tyrosine-protein phosphatase sigma, Receptor-type tyrosine-protein phosphatase S, R PTP sigma, R PTP S, PTPSIGMA

验证数据展示

  • WB analysis of HEK-293 using 29415-1-AP

    WB analysis of HEK-293 using 29415-1-AP

    WB result of PTPRS antibody (29415-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPRS transfected HEK-293 cells.

  • WB analysis using 29415-1-AP

    WB analysis using 29415-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29415-1-AP (PTPRS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29415-1-AP

    IHC staining of mouse brain using 29415-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29415-1-AP (PTPRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29415-1-AP

    IHC staining of mouse brain using 29415-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29415-1-AP (PTPRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29415-1-AP targets PTPRS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PTPRS fusion protein Ag30152 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, S
Synonyms Receptor-type tyrosine-protein phosphatase sigma, Receptor-type tyrosine-protein phosphatase S, R PTP sigma, R PTP S, PTPSIGMA
Calculated Molecular Weight 217 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberNM_002850
Gene Symbol PTPRS
Gene ID (NCBI) 5802
RRIDAB_2918301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13332
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPRS, also known as R-PTP-sigma or PTPσ, is a member of the protein tyrosine phosphatase (PTP) family (PMID: 8524829). PTPs dephosphorylate phosphotyrosyl residues in proteins that are phosphorylated by protein-tyrosine kinases (PTKs) (PMID: 20838449). PTPs are essential regulators of signal transduction pathways and play important roles in regulating many cellular processes in conjunction with PTKs (PMID: 7834742). PTPRS contains an extracellular region, a single transmembrane segment and two tandem intracellular catalytic domains, and thus represents a receptor-type PTP. Full-length PTPRS is 217 kDa and has been reported to be proteolytically cleaved into a 140-kDa N-terminal subunit containing extracellular and transmembrane domains and a C-terminal subunit (PMID: 29915291). This antibody raised against 825-1000 aa of human PTPRS detects endogenous PTPRS with an apparent molecular weight of 140 kDa.

实验方案

Product Specific Protocols
WB protocol for PTPRS antibody 29415-1-APDownload protocol
IHC protocol for PTPRS antibody 29415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols