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验证数据展示

  • WB analysis of HEK-293 using 29415-1-AP

    WB analysis of HEK-293 using 29415-1-AP

    WB result of PTPRS antibody (29415-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPRS transfected HEK-293 cells.

  • WB analysis using 29415-1-AP

    WB analysis using 29415-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29415-1-AP (PTPRS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29415-1-AP

    IHC staining of mouse brain using 29415-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29415-1-AP (PTPRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29415-1-AP

    IHC staining of mouse brain using 29415-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29415-1-AP (PTPRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

PTPRS Polyclonal antibody

PTPRS Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29415-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Receptor-type tyrosine-protein phosphatase sigma, Receptor-type tyrosine-protein phosphatase S, R PTP sigma, R PTP S, PTPSIGMA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29415-1-AP targets PTPRS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PTPRS fusion protein Ag30152 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, S
别名 Receptor-type tyrosine-protein phosphatase sigma, Receptor-type tyrosine-protein phosphatase S, R PTP sigma, R PTP S, PTPSIGMA
计算分子量 217 kDa
观测分子量140 kDa
GenBank蛋白编号NM_002850
基因名称 PTPRS
Gene ID (NCBI) 5802
RRIDAB_2918301
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13332
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPRS, also known as R-PTP-sigma or PTPσ, is a member of the protein tyrosine phosphatase (PTP) family (PMID: 8524829). PTPs dephosphorylate phosphotyrosyl residues in proteins that are phosphorylated by protein-tyrosine kinases (PTKs) (PMID: 20838449). PTPs are essential regulators of signal transduction pathways and play important roles in regulating many cellular processes in conjunction with PTKs (PMID: 7834742). PTPRS contains an extracellular region, a single transmembrane segment and two tandem intracellular catalytic domains, and thus represents a receptor-type PTP. Full-length PTPRS is 217 kDa and has been reported to be proteolytically cleaved into a 140-kDa N-terminal subunit containing extracellular and transmembrane domains and a C-terminal subunit (PMID: 29915291). This antibody raised against 825-1000 aa of human PTPRS detects endogenous PTPRS with an apparent molecular weight of 140 kDa.

实验方案

Product Specific Protocols
WB protocol for PTPRS antibody 29415-1-APDownload protocol
IHC protocol for PTPRS antibody 29415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols