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验证数据展示

  • WB analysis using 23786-1-AP

    WB analysis using 23786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23786-1-AP (PTPRU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 23786-1-AP

    IF Staining of U-251 using 23786-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PTPRU antibody (23786-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

PTPRU Polyclonal antibody

PTPRU Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23786-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

PTP, PCP2, PCP 2, hPTP J, GLEPP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, U-251 cells
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23786-1-AP targets PTPRU in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PTPRU fusion protein Ag20667 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, U
别名 PTP, PCP2, PCP 2, hPTP J, GLEPP1
计算分子量 1446 aa, 162 kDa
观测分子量170 kDa
GenBank蛋白编号BC146655
基因名称 PTPRU
Gene ID (NCBI) 10076
RRIDAB_3669416
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ92729
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPRU (protein tyrosine phosphatase, receptor type, U) is also named as PCP-2,PTP-J, PTP pi, PTP-RO and R-PTP-psi. Protein tyrosine phosphatase plays a crucial role in the regulation of signal transduction during diverse cell functions, including cell activation, proliferation, differentiation, survival, and metabolic homeostasis. PTPases are classified into two groups, membrane-spanning receptor-type (R-PTPases) and cytosolictype. The human PTPRU cDNA clone encodes a polypeptide of 1,439 amino acids and the predicted molecular mass of the PTPRU protein is 162 kDa. It has reported that SCF induced tyrosine phosphorylation of the full-length PTPRU (190 kDa) and the proteolytic forms of PTPRU (100 and 80 kD). A non-tyrosine phosphorylated 73 kDa band was detected in addition to those of 190, 90, and 80 kDa. (PMID: 10397721).

实验方案

Product Specific Protocols
WB protocol for PTPRU antibody 23786-1-APDownload protocol
IF protocol for PTPRU antibody 23786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols