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PTRF Polyclonal antibody

PTRF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 18892-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

FKSG13, Cavin-1, Cavin 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHT-1080 cells, rat heart tissue, mouse heart tissue, mouse lung tissue
Positive IP detected inHT-1080 cells
Positive IHC detected inhuman normal colon, mouse brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PTRF Ready-To-Use IHC Kit. PTRF primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18892-1-AP targets PTRF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, monkey
免疫原 PTRF fusion protein Ag13486 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase I and transcript release factor
别名 FKSG13, Cavin-1, Cavin 1
计算分子量 43 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC008849
基因名称 PTRF
Gene ID (NCBI) 284119
RRIDAB_10596795
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6NZI2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Polymerase I and transcript release factor(PTRF) is an essential factor in the biogenesis of caveolae, which involve in numerous process, such as signal transduction and membrane and lipid trafficking. PTRF can terminate transciption of RNA Pol I, which involves pausing of transcription by TTF1, and dissocaition of the transcription complex, release the complex from the template. The calculated molecular weight of PTRF is 43 kDa, but modified PTRF is about 50-55kDa. (PMID: 11139612 )

实验方案

Product Specific Protocols
WB protocol for PTRF antibody 18892-1-APDownload protocol
IHC protocol for PTRF antibody 18892-1-APDownload protocol
IF protocol for PTRF antibody 18892-1-APDownload protocol
IP protocol for PTRF antibody 18892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

ACS Nano

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Authors - Zhiwei Liao
humanWB,IF

Sci Adv

Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.

Authors - Virginia Andrade
  • KO Validated
humanWB,IF,IP

Dev Cell

Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2

Authors - Yeping Wu
humanWB,IHC

Autophagy

CAV1-CAVIN1-LC3B-mediated autophagy regulates high glucose-stimulated LDL transcytosis.

Authors - Xiangli Bai
  • KD Validated
mouseWB

Proc Natl Acad Sci U S A

Inducible Fgf13 ablation enhances caveolae-mediated cardioprotection during cardiac pressure overload.

Authors - Eric Q Wei
mouseIF

Theranostics

PTRF/Cavin-1 enhances chemo-resistance and promotes temozolomide efflux through extracellular vesicles in glioblastoma.

Authors - Eryan Yang
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