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PTRH2 Polyclonal antibody

PTRH2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 51006-2-AP

Print datasheet

Synonyms

BIT1, CGI 147, peptidyl tRNA hydrolase 2, PTH 2, PTH2, PTRH2

验证数据展示

  • WB analysis using 51006-2-AP

    WB analysis using 51006-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

  • IHC staining of human prostate cancer using 51006-2-AP

    IHC staining of human prostate cancer using 51006-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 51006-2-AP

    IHC staining of human prostate cancer using 51006-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 51006-2-AP

    IF Staining of HEK-293 using 51006-2-AP

    Immunofluorescent analysis of HEK-293 cells using 51006-2-AP (PTRH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, Jurkat cells, MCF-7 cells, Raji cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51006-2-AP targets PTRH2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PTRH2 fusion protein Ag0839 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidyl-tRNA hydrolase 2
Synonyms BIT1, CGI 147, peptidyl tRNA hydrolase 2, PTH 2, PTH2, PTRH2
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC006807
Gene Symbol PTRH2
Gene ID (NCBI) 51651
RRIDAB_2173280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3E5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTRH2 antibody 51006-2-APDownload protocol
IHC protocol for PTRH2 antibody 51006-2-APDownload protocol
IF protocol for PTRH2 antibody 51006-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols