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  • Featured Product
  • KD/KO Validated

Securin Polyclonal antibody

Securin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 18040-1-AP

Print datasheet

Synonyms

EAP1, Esp1 associated protein, HPTTG, pituitary tumor transforming 1, PTTG, PTTG1, Securin, Securin / PTTG1, Tumor transforming protein 1, TUTR1

验证数据展示

  • WB analysis using 18040-1-AP

    WB analysis using 18040-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18040-1-AP (Securin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human urothelial carcinoma using 18040-1-AP

    IHC staining of human urothelial carcinoma using 18040-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 18040-1-AP (Securin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTTG1/Securin antibody included
  • IF Staining of HepG2 using 18040-1-AP

    IF Staining of HepG2 using 18040-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Securin / PTTG1 antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of U2OS using 18040-1-AP

    IF Staining of U2OS using 18040-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Securin antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inDaudi cells, HepG2 cells, MDA-MB-231 cells
Positive IHC detected inhuman urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PTTG1/Securin Ready-To-Use IHC Kit. PTTG1/Securin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18040-1-AP targets Securin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen Securin fusion protein Ag12670 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pituitary tumor-transforming 1
Synonyms EAP1, Esp1 associated protein, HPTTG, pituitary tumor transforming 1, PTTG, PTTG1, Securin, Securin / PTTG1, Tumor transforming protein 1, TUTR1
Calculated Molecular Weight 202 aa, 22 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC101834
Gene Symbol Securin
Gene ID (NCBI) 9232
RRIDAB_2173410
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95997
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.

实验方案

Product Specific Protocols
WB protocol for Securin antibody 18040-1-APDownload protocol
IHC protocol for Securin antibody 18040-1-APDownload protocol
IF protocol for Securin antibody 18040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Front Immunol

Identification and validation of tryptophan-related gene signatures to predict prognosis and immunotherapy response in lung adenocarcinoma reveals a critical role for PTTG1

Authors - Ziqiang Wang
View Article
mouseIHC,IF

Cell Mol Life Sci

Microglial activation in an amyotrophic lateral sclerosis-like model caused by Ranbp2 loss and nucleocytoplasmic transport impairment in retinal ganglion neurons.

Authors - Kyoung-In Cho
View Article
humanWB

Sci Rep

APC/C(CDC20) and APC/C play pivotal roles in the process of embryonic development in Artemia sinica.

Authors - Mengchen Zhang
View Article
mouseWB,IF

Cell Cycle

Chronic restraint stress disturbs meiotic resumption through APC/C-mediated cyclin B1 excessive degradation in mouse oocytes.

Authors - Junyan Sun
View Article
mouseIF

Cryobiology

Melatonin promotes in vitro maturation of vitrified-warmed mouse GV oocytes potentially by modulating MAD2 protein expression of SAC component through MTRs.

Authors - Jinyu Yang
View Article
humanWB

Open Med (Wars)

The Pseudogene PTTG3P Promotes Cell Migration and Invasion in Esophageal Squamous Cell Carcinoma.

Authors - Zhenhua Zhang
  • KD Validated
View Article