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  • Featured Product
  • KD/KO Validated

PUF60 Polyclonal antibody, PBS Only

PUF60 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10810-1-PBS

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Synonyms

验证数据展示

  • WB analysis of HeLa using 10810-1-AP (same clone as 10810-1-PBS)

    WB analysis of HeLa using 10810-1-AP (same clone as 10810-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10810-1-AP (same clone as 10810-1-PBS)

    IHC staining of human colon cancer using 10810-1-AP (same clone as 10810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PUF60 antibody included
  • IHC staining of human breast cancer using 10810-1-AP (same clone as 10810-1-PBS)

    IHC staining of human breast cancer using 10810-1-AP (same clone as 10810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PUF60 antibody included
Planning an IHC experiment? We recommend our IHCeasy PUF60 Ready-To-Use IHC Kit. PUF60 primary antibody included.

产品信息

10810-1-PBS targets PUF60 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PUF60 fusion protein Ag1053 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name poly-U binding splicing factor 60KDa
Synonyms
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC008875
Gene Symbol PUF60
Gene ID (NCBI) 22827
RRIDAB_2173583
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHX1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.