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PUF60 Monoclonal antibody

PUF60 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A3A1

Cat No : 68533-1-Ig

Print datasheet

Synonyms

FBP interacting repressor, FIR, FLJ31379, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1

验证数据展示

  • WB analysis using 68533-1-Ig

    WB analysis using 68533-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 68533-1-Ig

    WB analysis of mouse testis using 68533-1-Ig

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMCF-7 cells, mouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy PUF60 Ready-To-Use IHC Kit. PUF60 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68533-1-Ig targets PUF60 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit
Immunogen PUF60 fusion protein Ag33930 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name poly-U binding splicing factor 60KDa
Synonyms FBP interacting repressor, FIR, FLJ31379, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC008875
Gene Symbol PUF60
Gene ID (NCBI) 22827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UHX1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.

实验方案

Product Specific Protocols
WB protocol for PUF60 antibody 68533-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols