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PUF60 Monoclonal antibody, PBS Only

PUF60 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A3A1

Cat No : 68533-1-PBS

Print datasheet

Synonyms

FBP interacting repressor, FIR, FLJ31379, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1

验证数据展示

  • WB analysis using 68533-1-Ig (same clone as 68533-1-PBS)

    WB analysis using 68533-1-Ig (same clone as 68533-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68533-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse testis using 68533-1-Ig (same clone as 68533-1-PBS)

    WB analysis of mouse testis using 68533-1-Ig (same clone as 68533-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68533-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PUF60 Ready-To-Use IHC Kit. PUF60 primary antibody included.

产品信息

68533-1-PBS targets PUF60 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit
Immunogen PUF60 fusion protein Ag33930 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name poly-U binding splicing factor 60KDa
Synonyms FBP interacting repressor, FIR, FLJ31379, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC008875
Gene Symbol PUF60
Gene ID (NCBI) 22827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UHX1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.