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- KD/KO Validated
PUM1 Polyclonal antibody
PUM1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
The immunogen of 26256-1-AP is PUM1 Fusion Protein expressed in E. coli.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | PUM1 fusion protein Ag24651 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | pumilio homolog 1 (Drosophila) |
| Synonyms | HSPUM, KIAA0099, PUM1, PUMH, PUMH1, Pumilio 1, Pumilio homolog 1, pumilio homolog 1 (Drosophila), PUML1 |
| Calculated Molecular Weight | 1186 aa, 126 kDa |
| Observed Molecular Weight | 130-140 kDa |
| GenBank Accession Number | BC013398 |
| Gene Symbol | PUM1 |
| Gene ID (NCBI) | 9698 |
| RRID | AB_2880448 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14671 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PUM1, also named as Pumilio homolog 1, is a 1186 amino acid protein, which is expressed in brain, heart, kidney, muscle, intestine and stomach. PUM1 as sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. PUM1 binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). The calculated molecular weight of PUM1 is 126 kDa, but the modified PUM1 is about 130-140 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PUM1 antibody 26256-1-AP | Download protocol |
| IF protocol for PUM1 antibody 26256-1-AP | Download protocol |
| IP protocol for PUM1 antibody 26256-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun PUM1 promotes ovarian cancer proliferation, migration and invasion.
| ||
Carcinogenesis PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway. |