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验证数据展示

  • WB analysis using 84399-3-RR

    WB analysis using 84399-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84399-3-RR (PURG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84399-3-RR

    IF Staining of HeLa using 84399-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PURG antibody (84399-3-RR, Clone: 241504E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84399-3-RR

    Affinity and Kinetic Characterization of 84399-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84399-3-RR against Human PURG were performed. The affinity constant is 0.328 nM.

PURG Recombinant antibody

PURG Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84399-3-RR

产品说明书

CloneNo. 241504E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

PURG B, PURG A, 241504E7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84399-3-RR targets PURG in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PURG fusion protein Ag15820 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 purine-rich element binding protein G
别名 PURG B, PURG A, 241504E7
计算分子量 347 aa, 40 kDa
观测分子量40 kDa
GenBank蛋白编号BC106708
基因名称 PURG
Gene ID (NCBI) 29942
RRIDAB_3671929
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UJV8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PURG antibody 84399-3-RRDownload protocol
IF protocol for PURG antibody 84399-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols