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Nectin-1/PVRL1 Polyclonal antibody

Nectin-1/PVRL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24713-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (2)

CD111, CLPED1, ED4, Herpes virus entry mediator C, Herpesvirus entry mediator C, Herpesvirus Ig like receptor, HIgR, HVEC, nectin 1, Nectin-1, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK 12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, SKOV-3 cells, SH-SY5Y cells, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24713-1-AP targets Nectin-1/PVRL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, pig
免疫原 Nectin-1/PVRL1 fusion protein Ag20074 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poliovirus receptor-related 1 (herpesvirus entry mediator C)
别名 CD111, CLPED1, ED4, Herpes virus entry mediator C, Herpesvirus entry mediator C, Herpesvirus Ig like receptor, HIgR, HVEC, nectin 1, Nectin-1, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK 12
计算分子量 517 aa, 57 kDa
观测分子量90 kDa, 57 kDa
GenBank蛋白编号BC104948
基因名称 Nectin-1
Gene ID (NCBI) 5818
RRIDAB_2879685
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15223
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nectins are a family of immunoglobulin-like cell adhesion molecules comprising four members, nectin-1 to nectin-4, each consisting of an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain (PMID: 12901789, 18703497). Nectins are involved in cell adhesion, migration, polarization, morphogenesis, proliferation, differentiation, and survival. Nectin-1, also known as PVRL1, HVEC and CD111, is widely expressed in a variety of cell types including epithelial cells, neurons, and fibroblasts (PMID: 12901789). It promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Nectin-1 acts as a receptor for several alphaherpesviruses, including herpes simplex viruses (HSV) 1 and 2, porcine pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1) (PMID: 9616127). Nectin-1 is a glycoprotein, the glycosylated form has a larger apparent molecular weight than the calculated molecular weight of 57 kDa (PMID: 12376527; 15731277).

实验方案

Product Specific Protocols
WB protocol for Nectin-1/PVRL1 antibody 24713-1-APDownload protocol
IHC protocol for Nectin-1/PVRL1 antibody 24713-1-APDownload protocol
IF protocol for Nectin-1/PVRL1 antibody 24713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Pathog

Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 1.

Authors - Hiroki Takeuchi
humanIHC,WB

Front Oncol

High expression of nectin-1 indicates a poor prognosis and promotes metastasis in hepatocellular carcinoma

Authors - Xuequan Wang
  • KD Validated
mouseIHC

Mol Ther Oncolytics

Oncolytic activity of naturally attenuated herpes-simplex virus HF10 against an immunocompetent model of oral carcinoma.

Authors - Gaku Takano
humanIHC

Heliyon

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study

Authors - Lingyan Chen

bioRxiv

Herpes Simplex Virus 1 Envelope Glycoprotein C Shields Glycoprotein D to Protect Virions from Entry-Blocking Antibodies

Authors - McKenna A Hull

J Virol

Neuropilin-1 Facilitates Pseudorabies Virus Replication and Viral Glycoprotein B Promotes Its Degradation in a Furin-Dependent Manner

Authors - Meng Chen
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