验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue, rat testis tissue |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11213-1-AP targets Nectin 3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | Nectin 3 fusion protein Ag1712 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | poliovirus receptor-related 3 |
别名 | Nectin-3, PVRL3, PPR3, Poliovirus receptor-related protein 3, poliovirus receptor related 3 |
计算分子量 | 61 kDa |
观测分子量 | 70-80 kDa |
GenBank蛋白编号 | BC017572 |
基因名称 | Nectin-3 |
Gene ID (NCBI) | 25945 |
RRID | AB_2269095 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9NQS3 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for Nectin 3 antibody 11213-1-AP | Download protocol |
IHC protocol for Nectin 3 antibody 11213-1-AP | Download protocol |
IP protocol for Nectin 3 antibody 11213-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Commun (Lond) ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin-3 and NRXN3. | ||
Front Microbiol Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B. | ||
J Neurochem Characterization of nectin processing mediated by presenilin-dependent γ-secretase. | ||
Biomed Pharmacother Nectin-3 is a new biomarker that mediates the upregulation of MMP2 and MMP9 in ovarian cancer cells. | ||
Biol Reprod Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis. | ||
Nat Chem Biol Photoproximity labeling of endogenous receptors in the live mouse brain in minutes |