Nectin 3 Polyclonal antibody

Nectin 3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 11213-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (1)

Nectin-3, PVRL3, PPR3, Poliovirus receptor-related protein 3, poliovirus receptor related 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11213-1-AP targets Nectin 3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Nectin 3 fusion protein Ag1712 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poliovirus receptor-related 3
别名 Nectin-3, PVRL3, PPR3, Poliovirus receptor-related protein 3, poliovirus receptor related 3
计算分子量 61 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC017572
基因名称 Nectin-3
Gene ID (NCBI) 25945
RRIDAB_2269095
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQS3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Nectin 3 antibody 11213-1-APDownload protocol
IHC protocol for Nectin 3 antibody 11213-1-APDownload protocol
IP protocol for Nectin 3 antibody 11213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Commun (Lond)

ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin-3 and NRXN3.

Authors - Yin Zhao
humanWB

Front Microbiol

Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B.

Authors - Dennis Schöttelndreier
mouse,ratWB,IF

J Neurochem

Characterization of nectin processing mediated by presenilin-dependent γ-secretase.

Authors - Kim Jinsook J
humanIHC

Biomed Pharmacother

Nectin-3 is a new biomarker that mediates the upregulation of MMP2 and MMP9 in ovarian cancer cells.

Authors - Feixue Xu
mouseIF

Biol Reprod

Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis.

Authors - Przemysław Zakrzewski
humanWB,IF

Nat Chem Biol

Photoproximity labeling of endogenous receptors in the live mouse brain in minutes

Authors - Mikiko Takato
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