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PXMP3 Polyclonal antibody

PXMP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 22163-1-AP

Print datasheet

Synonyms

PAF 1, PAF1, Peroxin 2, Peroxisomal membrane protein 3, Peroxisome assembly factor 1, Peroxisome biogenesis factor 2, PEX2, PMP3, PMP35, PXMP3, RING finger protein 72, RNF72

验证数据展示

  • WB analysis of mouse liver using 22163-1-AP

    WB analysis of mouse liver using 22163-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22163-1-AP (PXMP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 22163-1-AP

    IHC staining of human liver using 22163-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22163-1-AP (PXMP3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 22163-1-AP

    IHC staining of human liver using 22163-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22163-1-AP (PXMP3 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse liver tissue, HeLa cells, mouse brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

22163-1-AP targets PXMP3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen PXMP3 fusion protein Ag17815 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peroxisomal membrane protein 3, 35kDa
Synonyms PAF 1, PAF1, Peroxin 2, Peroxisomal membrane protein 3, Peroxisome assembly factor 1, Peroxisome biogenesis factor 2, PEX2, PMP3, PMP35, PXMP3, RING finger protein 72, RNF72
Calculated Molecular Weight 305 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC000661
Gene Symbol PXMP3
Gene ID (NCBI) 5828
RRIDAB_2879014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28328
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PXMP3 antibody 22163-1-APDownload protocol
IHC protocol for PXMP3 antibody 22163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Rep

Modulation of the cell membrane lipid milieu by peroxisomal β-oxidation induces Rho1 signaling to trigger inflammatory responses.

Authors - Anu S Nath
View Article