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PXR Monoclonal antibody, PBS Only

PXR Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G3A7

Cat no : 67912-1-PBS

Print datasheet

Synonyms

NR1I2, Orphan nuclear receptor PXR, Orphan nuclear receptor PAR1, ONR1, BXR

验证数据展示

  • IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67912-1-Ig (PXR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 67912-1-Ig (same clone as 67912-1-PBS)

    FC experiment of MCF-7 using 67912-1-Ig (same clone as 67912-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PXR (67912-1-Ig, Clone:1G3A7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • WB analysis using 67912-1-Ig (same clone as 67912-1-PBS)

    WB analysis using 67912-1-Ig (same clone as 67912-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67912-1-Ig (PXR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67912-1-Ig (PXR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

产品信息

67912-1-PBS targets PXR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PXR fusion protein Ag7991 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear receptor subfamily 1, group I, member 2
Synonyms NR1I2, Orphan nuclear receptor PXR, Orphan nuclear receptor PAR1, ONR1, BXR
Calculated Molecular Weight 50 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC017304
Gene Symbol PXR
Gene ID (NCBI) 8856
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75469
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pregnane X receptor (PXR; NR1I2), a member of the nuclear receptor superfamily, plays a key role in the induction of genes involved in drug transport and metabolism. It modulates drug transport and metabolism through the regulation of target genes responsible for the transport and conversion of chemicals into metabolites that are more easily eliminated from the body [PMID: 22609277]. It is also emerging as an endobiotic receptor that regulates the inflammatory response. In addition, PXR acts as a transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds [PMID: 19297428].