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Conjugate
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验证数据展示

  • IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67912-1-Ig (PXR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 67912-1-Ig (same clone as 67912-1-PBS)

    FC experiment of MCF-7 using 67912-1-Ig (same clone as 67912-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PXR (67912-1-Ig, Clone:1G3A7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • WB analysis using 67912-1-Ig (same clone as 67912-1-PBS)

    WB analysis using 67912-1-Ig (same clone as 67912-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67912-1-Ig (PXR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    IHC staining of mouse kidney using 67912-1-Ig (same clone as 67912-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67912-1-Ig (PXR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67912-1-PBS in a different storage buffer formulation.

PXR Monoclonal antibody, PBS Only

PXR Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67912-1-PBS

产品说明书

CloneNo. 1G3A7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

NR1I2, Orphan nuclear receptor PXR, Orphan nuclear receptor PAR1, ONR1, BXR

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67912-1-PBS targets PXR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PXR fusion protein Ag7991 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear receptor subfamily 1, group I, member 2
别名 NR1I2, Orphan nuclear receptor PXR, Orphan nuclear receptor PAR1, ONR1, BXR
计算分子量 50 kDa
观测分子量50 kDa
GenBank蛋白编号BC017304
基因名称 PXR
Gene ID (NCBI) 8856
RRIDAB_2918667
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75469
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pregnane X receptor (PXR; NR1I2), a member of the nuclear receptor superfamily, plays a key role in the induction of genes involved in drug transport and metabolism. It modulates drug transport and metabolism through the regulation of target genes responsible for the transport and conversion of chemicals into metabolites that are more easily eliminated from the body [PMID: 22609277]. It is also emerging as an endobiotic receptor that regulates the inflammatory response. In addition, PXR acts as a transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds [PMID: 19297428].