ASC/TMS1 Polyclonal antibody
ASC/TMS1 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue, mouse thymus tissue, rat spleen tissue, rat thymus tissue |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
30641-1-AP targets ASC/TMS1 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | ASC/TMS1 fusion protein Ag32519 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PYD and CARD domain containing |
| Synonyms | PYCARD, TMS 1, PYD and CARD domain containing, masc, CARD5 |
| Calculated Molecular Weight | 21KD |
| Observed Molecular Weight | 21-25 kDa |
| GenBank Accession Number | NM_023258 |
| Gene Symbol | Pycard |
| Gene ID (NCBI) | 66824 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9EPB4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASC/TMS1 antibody 30641-1-AP | Download protocol |
| IF protocol for ASC/TMS1 antibody 30641-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |