PYCR2-specific Polyclonal antibody
PYCR2-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, L02 cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
55060-1-AP targets PYCR2-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | pyrroline-5-carboxylate reductase family, member 2 |
Synonyms | PYCR2, PYCR 2, P5CR2, P5CR 2, HLD10 |
Calculated Molecular Weight | 34 kDa |
Observed Molecular Weight | 27-31 kDa, 20 kDa |
GenBank Accession Number | NM_013328 |
Gene Symbol | PYCR2 |
Gene ID (NCBI) | 29920 |
RRID | AB_10888635 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96C36 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PYCR2-specific antibody 55060-1-AP | Download protocol |
IHC protocol for PYCR2-specific antibody 55060-1-AP | Download protocol |
IF protocol for PYCR2-specific antibody 55060-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Rep Proline restores mitochondrial function and reverses aging hallmarks in senescent cells | ||
Sci Rep Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure. | ||
Int J Mol Sci Deciphering the Effects of the PYCR Family on Cell Function, Prognostic Value, Immune Infiltration in ccRCC and Pan-Cancer
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