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PYCR2-specific Polyclonal antibody

PYCR2-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 55060-1-AP

Print datasheet

Synonyms

PYCR2, PYCR 2, P5CR2, P5CR 2, HLD10

验证数据展示

  • WB analysis of HeLa using 55060-1-AP

    WB analysis of HeLa using 55060-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55060-1-AP (PYCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 55060-1-AP

    IF Staining of HeLa using 55060-1-AP

    Immunofluorescent analysis of HeLa cells using 55060-1-AP (PYCR2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human stomach cancer using 55060-1-AP

    IHC staining of human stomach cancer using 55060-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55060-1-AP (PYCR2-specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, L02 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55060-1-AP targets PYCR2-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyrroline-5-carboxylate reductase family, member 2
Synonyms PYCR2, PYCR 2, P5CR2, P5CR 2, HLD10
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 27-31 kDa, 20 kDa
GenBank Accession NumberNM_013328
Gene Symbol PYCR2
Gene ID (NCBI) 29920
RRIDAB_10888635
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96C36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody is specific to PYCR2 and has no cross reaction to PYCR1.

实验方案

Product Specific Protocols
WB protocol for PYCR2-specific antibody 55060-1-APDownload protocol
IHC protocol for PYCR2-specific antibody 55060-1-APDownload protocol
IF protocol for PYCR2-specific antibody 55060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Cell Rep

Proline restores mitochondrial function and reverses aging hallmarks in senescent cells

Authors - Debanik Choudhury
View Article
humanWB

Sci Rep

Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.

Authors - Bobby Jones
View Article
humanWB

Int J Mol Sci

Deciphering the Effects of the PYCR Family on Cell Function, Prognostic Value, Immune Infiltration in ccRCC and Pan-Cancer

Authors - Hongquan Chen
  • KD Validated
View Article