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验证数据展示

  • WB analysis using 68488-1-Ig

    WB analysis using 68488-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68488-1-Ig (PYCR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68488-1-Ig

    WB analysis using 68488-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68488-1-Ig (PYCR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

PYCR2 Monoclonal antibody

PYCR2 Monoclonal Antibody for WB, ELISA
Cat No. 68488-1-Ig

产品说明书

CloneNo. 2G7D9

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

FLJ54750, P5C reductase 2, P5CR 2, P5CR2, PYCR2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, LNCaP cells, K-562 cells, THP-1 cells, HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68488-1-Ig targets PYCR2 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 PYCR2 fusion protein Ag10923 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 pyrroline-5-carboxylate reductase family, member 2
别名 FLJ54750, P5C reductase 2, P5CR 2, P5CR2, PYCR2
计算分子量 320 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC020553
基因名称 PYCR2
Gene ID (NCBI) 29920
RRIDAB_3085199
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96C36
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.

实验方案

Product Specific Protocols
WB protocol for PYCR2 antibody 68488-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols