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PYCR2 Monoclonal antibody

PYCR2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G7D9

Cat no : 68488-1-Ig

Print datasheet

Synonyms

FLJ54750, P5C reductase 2, P5CR 2, P5CR2, PYCR2

验证数据展示

  • WB analysis using 68488-1-Ig

    WB analysis using 68488-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68488-1-Ig (PYCR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68488-1-Ig

    WB analysis using 68488-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68488-1-Ig (PYCR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inJurkat cells, LNCaP cells, K-562 cells, THP-1 cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68488-1-Ig targets PYCR2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen PYCR2 fusion protein Ag10923 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pyrroline-5-carboxylate reductase family, member 2
Synonyms FLJ54750, P5C reductase 2, P5CR 2, P5CR2, PYCR2
Calculated Molecular Weight 320 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC020553
Gene Symbol PYCR2
Gene ID (NCBI) 29920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96C36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.

实验方案

Product Specific Protocols
WB protocol for PYCR2 antibody 68488-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols