验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells, mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
16008-1-AP targets PYCRL in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | PYCRL fusion protein Ag8716 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pyrroline-5-carboxylate reductase-like |
| 别名 | PYCR3, Pyrroline-5-carboxylate reductase-like protein, Pyrroline-5-carboxylate reductase 3, P5CR 3, P5C reductase 3 |
| 计算分子量 | 274 aa, 29 kDa |
| 观测分子量 | 29 kDa |
| GenBank蛋白编号 | BC007993 |
| 基因名称 | PYCRL |
| Gene ID (NCBI) | 65263 |
| RRID | AB_3669228 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q53H96 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Proline conversion into P5C is coupled to the P5C reduction into proline, catalyzed by P5C reductase. This enzyme has three isoforms: PYCR1/PYCR2, which are specific for the mitochondrial compartment and the PYCRL present in cytosol. The conversion of P5C to proline, and its transport between mitochondria and cytosol, are also coupled with glucose metabolism by the pentose phosphate pathway.(PMID: 35163433)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PYCRL antibody 16008-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.
| ||
Vet Microbiol Host factor cyclophilin B affects Orf virus replication by interacting with viral ORF058 protein. |