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PYGB Polyclonal antibody

PYGB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55380-1-AP

Print datasheet

Synonyms

phosphorylase, glycogen; brain, PYGB

验证数据展示

  • WB analysis of A549 using 55380-1-AP

    WB analysis of A549 using 55380-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 55380-1-AP

    WB analysis of MCF-7 using 55380-1-AP

    WB result of PYGB antibody (55380-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGB transfected MCF-7 cells.

  • IHC staining of mouse heart using 55380-1-AP

    IHC staining of mouse heart using 55380-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PYGB antibody included
  • IF Staining of A549 using 55380-1-AP

    IF Staining of A549 using 55380-1-AP

    Immunofluorescent analysis of A549 cells using 55380-1-AP (PYGB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, U-251 cells
Positive IHC detected inmouse heart tissue, mouse brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy PYGB Ready-To-Use IHC Kit. PYGB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

55380-1-AP targets PYGB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphorylase, glycogen; brain
Synonyms phosphorylase, glycogen; brain, PYGB
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 97-105 kDa
GenBank Accession NumberNM_002862
Gene Symbol PYGB
Gene ID (NCBI) 5834
RRIDAB_11182159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11216
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PYGB belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. This antibody is specific to PYGB. It has no cross reaction to PYGM and PYGL.

实验方案

Product Specific Protocols
WB protocol for PYGB antibody 55380-1-APDownload protocol
IHC protocol for PYGB antibody 55380-1-APDownload protocol
IF protocol for PYGB antibody 55380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Mol Neurosci

Brain-Type Glycogen Phosphorylase Is Crucial for Astrocytic Glycogen Accumulation in Chronic Social Defeat Stress-Induced Depression in Mice.

Authors - Yuanyuan Zhu
View Article
human

J Immunol

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues

Authors - Teresa W-M Fan
View Article