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PYGL Polyclonal antibody

PYGL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15851-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

Glycogen phosphorylase, liver form, EC:2.4.1.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse liver, rat liver
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue, mouse heart tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15851-1-AP targets PYGL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, squirrel
免疫原 PYGL fusion protein Ag8664 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphorylase, glycogen, liver
别名 Glycogen phosphorylase, liver form, EC:2.4.1.1
计算分子量 846 aa, 97 kDa
观测分子量 97 kDa
GenBank蛋白编号BC009895
基因名称 PYGL
Gene ID (NCBI) 5836
RRIDAB_2175014
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06737
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PYGL antibody 15851-1-APDownload protocol
IHC protocol for PYGL antibody 15851-1-APDownload protocol
IF protocol for PYGL antibody 15851-1-APDownload protocol
IP protocol for PYGL antibody 15851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

Targeting SLC7A11 improves efferocytosis by dendritic cells and wound healing in diabetes.

Authors - Sophia Maschalidi

Nat Med

PMID: 30150719

Authors - Roger W Hunter
humanWB

Cell Metab

Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.

Authors - Marion Curtis
human,mouseWB

Cell Metab

Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses.

Authors - Phyu M Thwe
mouseWB

J Clin Invest

Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice.

Authors - Miguel A Lanaspa
humanIHC,IF,WB

Int J Biol Sci

PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer

Authors - Qian Ji
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