验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, mouse liver, rat liver |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human kidney tissue, mouse heart tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15851-1-AP targets PYGL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, squirrel |
免疫原 | PYGL fusion protein Ag8664 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | phosphorylase, glycogen, liver |
别名 | Glycogen phosphorylase, liver form, EC:2.4.1.1 |
计算分子量 | 846 aa, 97 kDa |
观测分子量 | 97 kDa |
GenBank蛋白编号 | BC009895 |
基因名称 | PYGL |
Gene ID (NCBI) | 5836 |
RRID | AB_2175014 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P06737 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for PYGL antibody 15851-1-AP | Download protocol |
IHC protocol for PYGL antibody 15851-1-AP | Download protocol |
IF protocol for PYGL antibody 15851-1-AP | Download protocol |
IP protocol for PYGL antibody 15851-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature Targeting SLC7A11 improves efferocytosis by dendritic cells and wound healing in diabetes. | ||
Cell Metab Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis. | ||
Cell Metab Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses. | ||
J Clin Invest Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice. | ||
Int J Biol Sci PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer |