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Pregnancy Zone Protein Polyclonal antibody

Pregnancy Zone Protein Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21742-1-AP

Print datasheet

Synonyms

PZP, pregnancy-zone protein

验证数据展示

  • WB analysis using 21742-1-AP

    WB analysis using 21742-1-AP

    Human plasma were subjected to SDS PAGE followed by western blot with 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 21742-1-AP

    IHC staining of human placenta using 21742-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 21742-1-AP

    IHC staining of human placenta using 21742-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman plasma, human placenta tissue
Positive IHC detected inhuman placenta tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21742-1-AP targets Pregnancy Zone Protein in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Pregnancy Zone Protein fusion protein Ag16471 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pregnancy-zone protein
Synonyms PZP, pregnancy-zone protein
Calculated Molecular Weight 1482 aa, 164 kDa
Observed Molecular Weight 164 kDa
GenBank Accession NumberBC111756
Gene Symbol PZP
Gene ID (NCBI) 5858
RRIDAB_10732823
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pregnancy Zone Protein (PZP) is an immunosuppressive protein that is expressed by the placenta. The PZP is a thiol ester-containing alpha macroglobulin protein that controls the proteolytic pathways during pregnancy and contributes to maintaining the immunologically privileged status of the fetus. Recently PZP over-expression has been described as a novel biomarker for predicting the prognosis of hepatocellular carcinoma and its high expression has been reported in solid tumors.

实验方案

Product Specific Protocols
WB protocol for Pregnancy Zone Protein antibody 21742-1-APDownload protocol
IHC protocol for Pregnancy Zone Protein antibody 21742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Clin Proteomics

iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.

Authors - Hongmei Lu
View Article