Pan-cadherin Polyclonal antibody

Pan-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31313-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

Pan Cadherin, E-cadherin, Arc 1, Cadherin 1, Cadherin-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, HeLa cells, MCF-7 cells, C6 cells, mouse brain tissue, mouse heart tissue
Positive IHC detected inmouse colon tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31313-1-AP targets Pan-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cadherin 1, type 1, E-cadherin (epithelial)
别名 Pan Cadherin, E-cadherin, Arc 1, Cadherin 1, Cadherin-1
计算分子量97 kDa
观测分子量120-130 kDa
GenBank蛋白编号NM_004360
基因名称 E-cadherin
Gene ID (NCBI) 999
RRIDAB_3669943
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP12830
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in tissue morphogenesis and the maintenance of normal tissue architecture (PMID: 23885883). The classic cadherin family members include E-, N-, P-, R-, and B-cadherins. The classic cadherins have ectodomains composed of five extracellular cadherin (EC) repeats, a single transmembrane region, and a cytoplasmic domain. Structural characterization indicated that the known cadherins are glycoproteins ranging in their apparent molecular weights between 120 and 140 kDa (PMID: 2077036). This polyclonal antibody is raised against a synthetic peptide corresponding to a conserved region of human E-Cadherin.

实验方案

Product Specific Protocols
WB protocol for Pan-cadherin antibody 31313-1-APDownload protocol
IHC protocol for Pan-cadherin antibody 31313-1-APDownload protocol
IF protocol for Pan-cadherin antibody 31313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...