验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, MCF-7 cells, C6 cells, mouse brain tissue, mouse heart tissue |
Positive IHC detected in | mouse colon tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
31313-1-AP targets Pan-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cadherin 1, type 1, E-cadherin (epithelial) |
别名 | Pan Cadherin, E-cadherin, Arc 1, Cadherin 1, Cadherin-1 |
计算分子量 | 97 kDa |
观测分子量 | 120-130 kDa |
GenBank蛋白编号 | NM_004360 |
基因名称 | E-cadherin |
Gene ID (NCBI) | 999 |
RRID | AB_3669943 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity Purification |
UNIPROT ID | P12830 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in tissue morphogenesis and the maintenance of normal tissue architecture (PMID: 23885883). The classic cadherin family members include E-, N-, P-, R-, and B-cadherins. The classic cadherins have ectodomains composed of five extracellular cadherin (EC) repeats, a single transmembrane region, and a cytoplasmic domain. Structural characterization indicated that the known cadherins are glycoproteins ranging in their apparent molecular weights between 120 and 140 kDa (PMID: 2077036). This polyclonal antibody is raised against a synthetic peptide corresponding to a conserved region of human E-Cadherin.
实验方案
Product Specific Protocols | |
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WB protocol for Pan-cadherin antibody 31313-1-AP | Download protocol |
IHC protocol for Pan-cadherin antibody 31313-1-AP | Download protocol |
IF protocol for Pan-cadherin antibody 31313-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |