Parvalbumin Polyclonal antibody

Parvalbumin Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 29312-1-AP

Print datasheet

Synonyms

PVALB, Parvalbumin alpha



经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue, mouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29312-1-AP targets Parvalbumin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen Parvalbumin fusion protein Ag30189 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name parvalbumin
Synonyms PVALB, Parvalbumin alpha
Calculated Molecular Weight 12 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberNM_002854
Gene Symbol Parvalbumin
Gene ID (NCBI) 5816
RRIDAB_2918280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20472
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation.

实验方案

Product Specific Protocols
WB protocol for Parvalbumin antibody 29312-1-APDownload protocol
IHC protocol for Parvalbumin antibody 29312-1-APDownload protocol
IF protocol for Parvalbumin antibody 29312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC,IF

Front Mol Neurosci

PGC-1α regulates critical period onset/closure, mediating cortical plasticity

Authors - Wei-Jun Zhang
ratIHC

Transl Androl Urol

Whole body vibration training promotes proprioceptive pathway for the treatment of stress urinary incontinence in rats

Authors - Chengyu Zhou
mouseWB

Mol Neurobiol

Oxidative Stress-mediated Loss of Hippocampal Parvalbumin Interneurons Contributes to Memory Precision Decline After Acute Sleep Deprivation

Authors - Yu-Zhu Gao
mouseIF

Cell Rep Methods

A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution

Authors - Jingtan Zhu
mouseIF

STAR Protoc

Protocol for fine casting, imaging, and analysis of murine vascular networks with VALID

Authors - Jingtan Zhu
mouseWB

Sci Rep

Identification of mouse soleus muscle proteins altered in response to changes in gravity loading

Authors - Yoko Ino