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Paxillin Polyclonal antibody

Paxillin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 32083-1-AP

Print datasheet

Synonyms

PXN

验证数据展示

  • WB analysis using 32083-1-AP

    WB analysis using 32083-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32083-1-AP (Paxillin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, COLO 320 cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32083-1-AP targets Paxillin in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Paxillin fusion protein Ag33616 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name paxillin
Synonyms PXN
Calculated Molecular Weight68kd
Observed Molecular Weight 68 kDa
GenBank Accession NumberNM_001080855
Gene Symbol Paxillin
Gene ID (NCBI) 5829
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PXN (paxillin) is a 68 kDa scaffold protein that interacts with multiple structural and signaling proteins and regulates cell adhesion, migration, proliferation, and apoptosis. PXN is thought to play an important role in tumor migration, invasion, and metastasis (21045234). PXN has been identified as a direct substrate of protein tyrosine phosphatase receptor-type T (PTPRT), a potent tumor suppressor gene. Increased phospho-PXN at tyrosine residue 88 (Y88) has been found as a common feature of human colon cancers (20133777).

实验方案

Product Specific Protocols
WB protocol for Paxillin antibody 32083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols