验证数据展示
经过测试的应用
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells, HeLa cells, C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 3 publications below |
产品信息
22271-1-AP targets Pericentrin in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, canine |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pericentrin |
| 别名 | PCNT, Pericentrin-B, Pericentrin B |
| 计算分子量 | 378 kDa |
| GenBank蛋白编号 | NM_006031 |
| 基因名称 | Pericentrin |
| Gene ID (NCBI) | 5116 |
| RRID | AB_2879056 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95613 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCNT, also named as KIAA0402, PCNT2, Pericentrin-B and Kendrin, is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. PCNT plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). PCNT is a marker of centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2). The antibody is specific to PCNT, and it can recognize isoform 1 and 2.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Pericentrin antibody 22271-1-AP | Download protocol |
| IF protocol for Pericentrin antibody 22271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly. | ||
Cell Death Differ Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis | ||
Cell Rep Aurora A polyubiquitinates the BRCA1-interacting protein OLA1 to promote centrosome maturation |
