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Pericentrin Polyclonal antibody

Pericentrin Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27084-1-AP

Print datasheet

Synonyms

PCNT, Pericentrin-B, Pericentrin B

验证数据展示

  • IF Staining of HeLa using 27084-1-AP

    IF Staining of HeLa using 27084-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Pericentrin antibody (27084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human thyroid cancer using 27084-1-AP

    IHC staining of human thyroid cancer using 27084-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27084-1-AP (Pericentrin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 27084-1-AP

    IHC staining of human thyroid cancer using 27084-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27084-1-AP (Pericentrin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 27084-1-AP

    IF Staining of HeLa using 27084-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Pericentrin antibody (27084-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27084-1-AP targets Pericentrin in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Pericentrin fusion protein Ag25888 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pericentrin
Synonyms PCNT, Pericentrin-B, Pericentrin B
Calculated Molecular Weight 378 kDa
GenBank Accession NumberAK093923
Gene Symbol Pericentrin
Gene ID (NCBI) 5116
RRIDAB_3085924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCNT, also named as KIAA0402, PCNT2, Pericentrin-B and Kendrin, is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. PCNT plays a role, together with DISC1, in the microtubule network formation. PCNT is a marker of centrosome. Defects in PCNT are the cause of microcephalic osteodysplastic primordial dwarfism type 2 (MOPD2).

实验方案

Product Specific Protocols
IHC protocol for Pericentrin antibody 27084-1-APDownload protocol
IF protocol for Pericentrin antibody 27084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols